Accession	MF_00163
Dates	1-JUN-2001 (Created) 7-JUN-2008 (Last updated, Version 21)

Data class

Protein

Predictors

HAMAP; MF_00163; [distribution of match scores in UniProtKB];[seed alignment for MF_00163]

Identifier

DEF

Protein name

RecName: Full=Peptide deformylase; Short=PDF; EC=3.5.1.88; AltName: Full=Polypeptide deformylase;

Gene name

def

FUNCTION: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).

CATALYTIC ACTIVITY: Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide.

COFACTOR: Binds 1 Fe(2+) ion (By similarity).

SIMILARITY: Belongs to the polypeptide deformylase family.

Pfam	PF01327; Pep_deformylase; 1;
PRINTS	PR01576; PDEFORMYLASE; 1;
TIGRFAMs	TIGR00079; pept_deformyl; 1;

Protein biosynthesis, Hydrolase.

Iron, Metal-binding.

GO:0042586; Molecular function: peptide deformylase activity.
GO:0006412; Biological process: translation.

From: DEF_ECOLI (P0A6K3)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		134		134		By similarity		E
METAL		91		91		Iron (By similarity)		C		1
METAL		133		133		Iron (By similarity)		H		1
METAL		137		137		Iron (By similarity)		H		1

Size range:	136-232 amino acids
Related UniRules:	None
Template:	P0A6K3 (DEF_ECOLI); O31410 (DEF2_BACST); Q93LE9 (DEF_LEPIN); P68826 (DEF_STAAU); Q45495 (DEF2_BACSU); P43522 (DEF_THETH): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in ANASP, BACAN, BACCR, BACSU, BIFLO, BORBR, BORPA, BORPE, CLOAB, CLOPE, COREF, CORGL, COXBU, GLOVI, NITEU, PROMM, RALSO, RICCN, STRAW, STRCO, VIBCH, VIBVY, XANAC, XANCP
Plasmid encoded:	None
Comments:	Possible wrong start in a number of bacteria. Def-like proteins exist a number of bacteria and lack some active site and metal-binding residues.