[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Sprague-Dawley; TISSUE=Liver; Nishino H., Ishibashi T.; "Transmembrane configuration of sterol delta 7-reductase as a potential sterol sensing protein."; Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Sprague-Dawley; DOI=10.1074/jbc.274.21.14624; PubMed=10329655 [NCBI, ExPASy, EBI, Israel, Japan] Bae S.-H., Lee J.N., Fitzky B.U., Seong J., Paik Y.-K.; "Cholesterol biosynthesis from lanosterol. Molecular cloning, tissue distribution, expression, chromosomal localization, and regulation of rat 7-dehydrocholesterol reductase, a Smith-Lemli-Opitz syndrome-related protein."; J. Biol. Chem. 274:14624-14631(1999).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. DOI=10.1016/S0167-4781(02)00285-3; PubMed=12031495 [NCBI, ExPASy, EBI, Israel, Japan] Lee J.-N., Bae S.-H., Paik Y.-K.; "Structure and alternative splicing of the rat 7-dehydrocholesterol reductase gene."; Biochim. Biophys. Acta 1576:148-156(2002).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
CATALYTIC ACTIVITY: Cholesterol + NADP⁺ = cholesta-5,7-dien-3-beta-ol + NADPH.
PATHWAY: Steroid biosynthesis; cholesterol biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the ERG4/ERG24 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB016800; BAA34306.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF071500; AAD31383.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF272393; AAM45144.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF279892; AAK69490.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC081688; AAH81688.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_071784.1; -.

UniGene

Rn.228

3D structure databases

ModBase

Q9Z2Z8.

Organism-specific databases

RGD

621769; Dhcr7.

Gene expression databases

ArrayExpress

Q9Z2Z8; -.

GermOnline

ENSRNOG00000020776; Rattus norvegicus.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0047598;	Molecular function: 7-dehydrocholesterol reductase activity (inferred from electronic annotation from EC).
GO:0006695;	Biological process: cholesterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001171; ERG4_ERG24.
Graphical view of domain structure.

Pfam

PF01222; ERG4_ERG24; 1.
Pfam graphical view of domain structure.

PROSITE

PS01017; STEROL_REDUCT_1; 1.
PS01018; STEROL_REDUCT_2; 1.

ProtoNet

Q9Z2Z8.

Genome annotation databases

Ensembl

ENSRNOG00000020776; Rattus norvegicus. [Contig view]

GeneID

64191; -.

KEGG

rno:64191; -.

Phylogenomic databases

HOVERGEN

Q9Z2Z8; -.

Other

NextBio

612852; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Endoplasmic reticulum; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 471`	`471`	`7-dehydrocholesterol reductase.`	`PRO_0000207504`
`TRANSMEM`	`36 56`	`21`	`Potential.`
`TRANSMEM`	`95 115`	`21`	`Potential.`
`TRANSMEM`	`144 164`	`21`	`Potential.`
`TRANSMEM`	`173 193`	`21`	`Potential.`
`TRANSMEM`	`233 253`	`21`	`Potential.`
`TRANSMEM`	`262 282`	`21`	`Potential.`
`TRANSMEM`	`302 322`	`21`	`Potential.`
`TRANSMEM`	`327 347`	`21`	`Potential.`
`TRANSMEM`	`416 436`	`21`	`Potential.`

Sequence information

Length: 471 AA [This is the length of the unprocessed precursor]

Molecular weight: 54155 Da [This is the MW of the unprocessed precursor]

CRC64: EBF0CBC4F4222FDB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASKSQHNAS KAKNHNVKAE SQGQWGRAWE VDWFSLVSVI FLLLFAPFIV YYFIMACDQY 

        70         80         90        100        110        120 
SCSLTAPILD VATGRASLAD IWAKTPPVTA KAAQLYALWV SFQVLLYSWL PDFCHRFLPG 

       130        140        150        160        170        180 
YVGGVQEGAI TPAGIVNKYE VNGLQAWLIT HFLWFVNAYL LSWFSPTIIF DNWIPLLWCA 

       190        200        210        220        230        240 
NILGYAVSTF AMIKGYLFPT SAEDCKFTGN FFYNYMMGIE FNPRIGKWFD FKLFFNGRPG 

       250        260        270        280        290        300 
IVAWTLINLS FAAKQQELYG HVTNSMILVN VLQAIYVLDF FWNETWYLKT IDICHDHFGW 

       310        320        330        340        350        360 
YLGWGDCVWL PYLYTLQGLY LVYHPVQLST PNALGVLLLG LVGYYIFRMT NHQKDLFRRT 

       370        380        390        400        410        420 
DGHCLIWGKK PKAIECSYTS ADGLKHRSKL LVSGFWGVAR HFNYTGDLMG SLAYCLACGG 

       430        440        450        460        470 
GHLLPYFYII YMTILLTHRC LRDEHRCANK YGRDWERYVA AVPYRLLPGI F

Q9Z2Z8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools