[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=New Zealand white; TISSUE=Liver; Yamada M., Setoguchi Y., Setoguchi T.; "Delta4-3-oxosteroid 5beta-reductase."; Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
CATALYTIC ACTIVITY: 5-beta-cholestan-3-one + NADP⁺ = cholest-4-en-3-one + NADPH.
CATALYTIC ACTIVITY: 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP⁺ = cortisone.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the aldo/keto reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB023951; BAA82867.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001075466.1; -.

UniGene

Ocu.2365

3D structure databases

HSSP

P52895; 1J96. [HSSP ENTRY / PDB]

ModBase

Q9TV64.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0047787;	Molecular function: delta4-3-oxosteroid 5beta-reductase activity (inferred from electronic annotation from EC).
GO:0030573;	Biological process: bile acid catabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001395; Aldo/ket_red.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.100; Aldo/ket_red; 1.

PANTHER

PTHR11732; Aldo/ket_red; 1.

Pfam

PF00248; Aldo_ket_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00069; ALDKETRDTASE.

ProDom

PD000288; Aldo/ket_red; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00798; ALDOKETO_REDUCTASE_1; 1.
PS00062; ALDOKETO_REDUCTASE_2; 1.
PS00063; ALDOKETO_REDUCTASE_3; 1.

Genome annotation databases

GeneID

100008612; -.

Phylogenomic databases

HOVERGEN

Q9TV64; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Bile acid catabolism; Cytoplasm; Lipid metabolism; NADP; Oxidoreductase; Steroid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 326`	`326`	`3-oxo-5-beta-steroid 4-dehydrogenase.`	`PRO_0000124671`
`NP_BIND`	`169 170`	`2`	`NADP (By similarity).`
`NP_BIND`	`220 224`	`5`	`NADP (By similarity).`
`NP_BIND`	`273 283`	`11`	`NADP (By similarity).`
`ACT_SITE`	`58 58`		`Proton donor (By similarity).`
`BINDING`	`53 53`		`NADP (By similarity).`
`BINDING`	`193 193`		`NADP (By similarity).`
`BINDING`	`230 230`		`Substrate (By similarity).`

Sequence information

Length: 326 AA [This is the length of the unprocessed precursor]

Molecular weight: 37491 Da [This is the MW of the unprocessed precursor]

CRC64: DA7618FFC3F849AC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLSATNHRI PLGDGNSIPI IGLGTYSEPK TTPKGSCATS VKIAIDTGYR HIDGAYIYQN 

        70         80         90        100        110        120 
EHEVGETFRE KIAEGKVRRE DIFYCGKLWA TNHDPVMVRP TLERTLKVLK LDYIDLYIIE 

       130        140        150        160        170        180 
IPMAFKPGDV VYPRDENGKW LYHKTNLCAT WEALEACKDA GLVKSLGVSN FNRQQLELLL 

       190        200        210        220        230        240 
NKPGLKHKPV CNQVECHPYF TQPKLLKFCQ QHDIIIVAYS PLGTCRNPMW VNTSLPPLLK 

       250        260        270        280        290        300 
DTLLNSLGKK YKKTAAQIVL RFNVQRGVVV IPKSFNPERI KENFQIFDFS LTEEEMKDIE 

       310        320 
ALNKNVRYVE LLMWRDHPEY PFNDEY

Q9TV64 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools