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UniProtKB/Swiss-Prot entry Q9STX3


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GID2_ARATH
Primary accession number Q9STX3
Secondary accession number Q8LAQ7
Integrated into Swiss-Prot on November 22, 2005
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 47)
Name and origin of the protein
Protein name F-box protein GID2
Synonym Protein SLEEPY 1
Gene name
Name: GID2
Synonyms: SLY1
OrderedLocusNames: At4g24210
ORFNames: T22A6.40
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/47134; PubMed=10617198 [NCBI, ExPASy, EBI, Israel, Japan]
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.;
"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
Nature 402:769-777(1999).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
[4]
FUNCTION, AND TISSUE SPECIFICITY.
DOI=10.1105/tpc.010827; PubMed=12724538 [NCBI, ExPASy, EBI, Israel, Japan]
McGinnis K.M., Thomas S.G., Soule J.D., Strader L.C., Zale J.M., Sun T.-P., Steber C.M.;
"The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase.";
Plant Cell 15:1120-1130(2003).
[5]
FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF A SCF COMPLEX, INTERACTION WITH GAI AND RGA, AND MUTAGENESIS OF GLU-138.
DOI=10.1105/tpc.020958; PubMed=15155881 [NCBI, ExPASy, EBI, Israel, Japan]
Dill A., Thomas S.G., Hu J., Steber C.M., Sun T.-P.;
"The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation.";
Plant Cell 16:1392-1405(2004).
[6]
FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF A SCF COMPLEX WITH SKP1B AND CUL1, INTERACTION WITH SKP1A; SKP1B; GAI AND RGA, AND MUTAGENESIS OF GLU-138.
DOI=10.1105/tpc.021386; PubMed=15161962 [NCBI, ExPASy, EBI, Israel, Japan]
Fu X., Richards D.E., Fleck B., Xie D., Burton N., Harberd N.P.;
"The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates.";
Plant Cell 16:1406-1418(2004).
[7]
INTERACTION WITH GAI; RGA; RGL1 AND RGL3.
DOI=10.1104/pp.104.039578; PubMed=15173565 [NCBI, ExPASy, EBI, Israel, Japan]
Tyler L., Thomas S.G., Hu J., Dill A., Alonso J.M., Ecker J.R., Sun T.-P.;
"Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis.";
Plant Physiol. 135:1008-1019(2004).
[8]
FUNCTION.
DOI=10.1073/pnas.0404287101; PubMed=15308775 [NCBI, ExPASy, EBI, Israel, Japan]
Strader L.C., Ritchie S., Soule J.D., McGinnis K.M., Steber C.M.;
"Recessive-interfering mutations in the gibberellin signaling gene SLEEPY1 are rescued by overexpression of its homologue, SNEEZY.";
Proc. Natl. Acad. Sci. U.S.A. 101:12771-12776(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AL078637; CAB45056.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL161561; CAB79331.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT004192; AAO42210.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT005395; AAO63815.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY087672; AAM65209.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T09884; T09884.
RefSeq NP_194152.1; -.
UniGene At.43332
3D structure databases
ModBase Q9STX3.
Protein-protein interaction databases
IntAct Q9STX3; -.
Organism-specific databases
TAIR At4g24210; -.
Gene expression databases
GermOnline AT4G24210; Arabidopsis thaliana.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001810; F-box.
Graphical view of domain structure.
Pfam PF00646; F-box; 1.
Pfam graphical view of domain structure.
SMART SM00256; FBOX; 1.
SMART graphical view of domain structure.
PROSITE PS50181; FBOX; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9STX3.
Genome annotation databases
GeneID 828522; -.
GenomeReviews CT486007_GR; AT4G24210.
KEGG ath:AT4G24210; -.
NMPDR fig|3702.1.peg.20308; -.
Other
ProtoNet Q9STX3.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Gibberellin signaling pathway; Nucleus; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   151  151     F-box protein GID2. PRO_0000119962
DOMAIN   29    75  47     F-box. 
MUTAGEN   138   138        E->K: In gar2-1/sly1-d; gain of function allele that promotes plant growth by increasing the affinity with DELLA protein substrates. 
CONFLICT   3     3        R -> L (in Ref. 3; AAM65209). 
Sequence information
Length: 151 AA [This is the length of the unprocessed precursor] Molecular weight: 17455 Da [This is the MW of the unprocessed precursor] CRC64: 4404DE81E9DFDA9D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKRSTTDSDL AGDAHNETNK KMKSTEEEEI GFSNLDENLV YEVLKHVDAK TLAMSSCVSK 

        70         80         90        100        110        120 
IWHKTAQDER LWELICTRHW TNIGCGQNQL RSVVLALGGF RRLHSLYLWP LSKPNPRARF 

       130        140        150 
GKDELKLTLS LLSIRYYEKM SFTKRPLPES K 

Q9STX3 in FASTA format

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