FUNCTION: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).
CATALYTIC ACTIVITY: 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂.
COFACTOR: Thiamine pyrophosphate (By similarity).
SUBUNIT: Heterodimer of an alpha and a beta chain (By similarity).

Copyright

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Cross-references

Sequence databases

EMBL

AE004091; AAG05636.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

D83365; D83365.
S63478; S63478.

RefSeq

NP_250938.1; -.

3D structure databases

HSSP

P09061; 1QS0. [HSSP ENTRY / PDB]

SMR

Q9I1M1; 15-350.

ModBase

Q9I1M1.

Enzyme and pathway databases

BioCyc

PAER208964:PA2248-MON; -.

Organism-specific databases

PseudoCAP

PA2248; -.

Ontologies

GO:0003863;	Molecular function: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR005476; Transketo_C.
IPR005475; Transketo_Cen_R.
IPR015941; Transketolase_C-like.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.920; Transketo_C_like; 1.

Pfam

PF02779; Transket_pyr; 1.
PF02780; Transketolase_C; 1.
Pfam graphical view of domain structure.

ProtoNet

Q9I1M1.

Genome annotation databases

GeneID

879226; -.

GenomeReviews

AE004091_GR; PA2248.

KEGG

pae:PA2248; -.

Phylogenomic databases

HOGENOM

Q9I1M1; -.

Genome annotation databases

CMR

Q9I1M1; PA2248.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Oxidoreductase; Thiamine pyrophosphate.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 350`	`350`		`2-oxoisovalerate dehydrogenase subunit beta.`	`PRO_0000287783`

Sequence information

Length: 350 AA [This is the length of the unprocessed precursor]

Molecular weight: 38325 Da [This is the MW of the unprocessed precursor]

CRC64: D7983A05A9B3EB0A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNAMNPQHEN AQTVTSMTMI QALRSAMDIM LERDDDVVVF GQDVGYFGGV FRCTEGLQKK 

        70         80         90        100        110        120 
YGTSRVFDAP ISESGIIGAA VGMGAYGLRP VVEIQFADYV YPASDQLISE AARLRYRSAG 

       130        140        150        160        170        180 
DFIVPMTVRM PCGGGIYGGQ THSQSPEAMF TQVCGLRTVM PSNPYDAKGL LIACIENDDP 

       190        200        210        220        230        240 
VIFLEPKRLY NGPFDGHHDR PVTPWSKHPA SQVPDGYYKV PLDKAAIVRP GAALTVLTYG 

       250        260        270        280        290        300 
TMVYVAQAAA DETGLDAEII DLRSLWPLDL ETIVASVKKT GRCVIAHEAT RTCGFGAELM 

       310        320        330        340        350 
SLVQEHCFHH LEAPIERVTG WDTPYPHAQE WAYFPGPARV GAAFKRVMEV

Q9I1M1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools