NUCLEOTIDE SEQUENCE [MRNA], SELF-ASSOCIATION, INTERACTION WITH SHANK1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley;
TISSUE=Brain;
DOI=10.1006/mcne.2000.0940; PubMed=11178875 [NCBI, ExPASy, EBI, Israel, Japan]
Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E.;
"Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins.";
Mol. Cell. Neurosci. 17:385-397(2001).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Heart;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: May have a role in normal immune development and control of inflammation (By similarity).
SUBUNIT: Self-associates. Interacts with SHANK1.
INTERACTION:
Self; NbExp=1; IntAct=EBI-1394695, EBI-1394695;
P31016:Dlg4; NbExp=1; IntAct=EBI-1394695, EBI-375655;
P97879:Grip1; NbExp=1; IntAct=EBI-1394695, EBI-936113;
Q9WV48:Shank1; NbExp=4; IntAct=EBI-1394695, EBI-80909;
SUBCELLULAR LOCATION: Cytoplasm. Note=Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
TISSUE SPECIFICITY: Expressed in brain, spleen, lung, heart, skeletal muscle, kidney and testis (at protein level). Expressed in heart and testis.
SIMILARITY: Contains 1 RanBP2-type zinc finger.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF203906; AAG43482.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC083553; AAH83553.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_112415.1; -.

UniGene

Rn.38393

3D structure databases

ModBase

Q9EQL9.

Protein-protein interaction databases

IntAct

Q9EQL9; -.

Organism-specific databases

RGD

631353; Sharpin.

Gene expression databases

ArrayExpress

Q9EQL9; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0042802;	Molecular function: identical protein binding (inferred from physical interaction from IntAct).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001876; Znf_RanBP2.
Graphical view of domain structure.

SMART

SM00547; ZnF_RBZ; 1.
SMART graphical view of domain structure.

PROSITE

PS01358; ZF_RANBP2_1; 1.
PS50199; ZF_RANBP2_2; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q9EQL9.

Genome annotation databases

Ensembl

ENSRNOG00000012812; Rattus norvegicus. [Contig view]

GeneID

81859; -.

KEGG

rno:81859; -.

Phylogenomic databases

HOVERGEN

Q9EQL9; -.

Other

NextBio

615783; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Metal-binding; Zinc; Zinc-finger.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 381`	`381`	`Sharpin.`	`PRO_0000280636`
`ZN_FING`	`342 371`	`30`	`RanBP2-type.`
`REGION`	`1 177`	`177`	`Self-association.`
`REGION`	`172 305`	`134`	`Interaction with SHANK1.`

Sequence information

Length: 381 AA [This is the length of the unprocessed precursor]

Molecular weight: 40287 Da [This is the MW of the unprocessed precursor]

CRC64: 8D9A7D7D23665567 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSPPAGGAAA AADPASPVVL LAVQAAVRLL GAGHEDEAQL RKLQLKADPE RPGRFRLGLL 

        70         80         90        100        110        120 
GIEPGAVSLE WPLESICYTI RGPNQHELQP PPGGPGTFSV HFLNSEEAQQ WAALVRDATA 

       130        140        150        160        170        180 
EGQNGNDSTA PVPTPAMCPT SPPCSSVTPT PKATQPEMDL PQGSGNLKKE ELATHLAQAI 

       190        200        210        220        230        240 
AGGDEKAAAQ VAAILAQHHV ALNVQLLEAW FPRGPIRLQV TVEDATSVLS SSSSAHVSLQ 

       250        260        270        280        290        300 
IHPHCSIAAL QEQVFSEFGF PPAVQRWVIG RCLCMPERSL ASYGVSQDGD PAFLYLLSAP 

       310        320        330        340        350        360 
REVSGHSPQH SKMDRKLGCL FPQSLELPHN LQASSSSLPS PPQPGWSCPS CTFINASNRP 

       370        380 
GCEMCSTQRP CAWDPLTATS T

Q9EQL9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools