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UniProtKB/Swiss-Prot entry Q8R1I2


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GRP_MOUSE
Primary accession number Q8R1I2
Secondary accession number Q3UTL2
Integrated into Swiss-Prot on November 28, 2006
Sequence was last modified on June 1, 2002 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 39)
Name and origin of the protein
Protein name Gastrin-releasing peptide [Precursor]
Synonym GRP
Contains Neuromedin-C
     (GRP-10)
Gene name
Name: Grp
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Brain cortex;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=FVB/N;
TISSUE=Colon;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
TISSUE SPECIFICITY, AND FUNCTION IN CONTROL OF FEAR.
DOI=10.1016/S0092-8674(02)01116-9; PubMed=12526815 [NCBI, ExPASy, EBI, Israel, Japan]
Shumyatsky G.P., Tsvetkov E., Malleret G., Vronskaya S., Hatton M., Hampton L., Battey J.F., Dulac C., Kandel E.R., Bolshakov V.Y.;
"Identification of a signaling network in lateral nucleus of amygdala important for inhibiting memory specifically related to learned fear.";
Cell 111:905-918(2002).
Comments
  • FUNCTION: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP-receptor gene expression, long-term potentiation, and amygdala-dependent memory for fear.
  • SUBCELLULAR LOCATION: Secreted.
  • TISSUE SPECIFICITY: Highly expressed both in the lateral nucleus of the amygdala, and in regions sending synaptic projections to the lateral nucleus.
  • SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AK133508; BAE21694.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK139345; BAE23968.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC024515; AAH24515.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_778177.1; -.
UniGene Mm.20298
3D structure databases
ModBase Q8R1I2.
Organism-specific databases
MGI MGI:95833; Grp.
Gene expression databases
ArrayExpress Q8R1I2; -.
CleanEx MM_GRP; -.
GermOnline ENSMUSG00000024517; Mus musculus.
Family and domain databases
InterPro IPR000874; Bombesin.
IPR015674; Gastrin-RP.
Graphical view of domain structure.
PANTHER PTHR16866; Gastrin-RP; 1.
Pfam PF02044; Bombesin; 1.
Pfam graphical view of domain structure.
PROSITE PS00257; BOMBESIN; 1.
BLOCKS Q8R1I2.
Genome annotation databases
Ensembl ENSMUSG00000024517; Mus musculus. [Contig view]
GeneID 225642; -.
KEGG mmu:225642; -.
Phylogenomic databases
HOVERGEN Q8R1I2; -.
Other
SOURCE Grp; Mus musculus.
ProtoNet Q8R1I2.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amidation; Cleavage on pair of basic residues; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    23  23     By similarity. 
PEPTIDE   24    52  29     Gastrin-releasing peptide. PRO_0000262472
PEPTIDE   43    52  10     Neuromedin-C. PRO_0000262473
PROPEP   56   146  91     By similarity. PRO_0000262474
MOD_RES   52    52        Methionine amide (By similarity). 
CONFLICT   134   134        L -> Q (in Ref. 1; BAE23968). 
Sequence information
Length: 146 AA [This is the length of the unprocessed precursor] Molecular weight: 15659 Da [This is the MW of the unprocessed precursor] CRC64: 83FD03C40E670CA5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRGSELSLLL LALVLCQAPR GPAAPVSTGA GGGTVLAKMY PRGSHWAVGH LMGKKSTDES 

        70         80         90        100        110        120 
PSLYAADRDG LKEQLRGYVR WEEAARDLLD LLEAAGNQSH QPPQHPPLSL QPTWDPEDGS 

       130        140 
YFNDVQTAKL VDSLLQVLKE KGGTAS 

Q8R1I2 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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