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UniProtKB/Swiss-Prot entry Q64380

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SARDH_RAT
Primary accession number Q64380
Secondary accession number O88499
Integrated into Swiss-Prot on September 13, 2004
Sequence was last modified on August 16, 2004 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 56)
Name and origin of the protein
Protein name Sarcosine dehydrogenase, mitochondrial [Precursor]
Synonyms SarDH
EC 1.5.99.1
Gene name
Name: Sardh
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 192-207, FAD-BINDING, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley, and Wistar;
TISSUE=Liver;
PubMed=9839943 [NCBI, ExPASy, EBI, Israel, Japan]
Bergeron F., Otto A., Blache P., Day R., Denoroy L., Brandsch R., Bataille D.;
"Molecular cloning and tissue distribution of rat sarcosine dehydrogenase.";
Eur. J. Biochem. 257:556-561(1998).
[2]
 PROTEIN SEQUENCE OF 102-115, AND FAD-BINDING.
TISSUE=Liver;
PubMed=4055729 [NCBI, ExPASy, EBI, Israel, Japan]
Cook R.J., Misono K.S., Wagner C.;
"The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria.";
J. Biol. Chem. 260:12998-13002(1985).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF067650; AAD03414.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U62481; AAB03674.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L79910; AAB04667.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_446116.1; -.
UniGene Rn.89832
3D structure databases
ModBase Q64380.
Organism-specific databases
RGD 621125; Sardh.
Gene expression databases
ArrayExpress Q64380; -.
GermOnline ENSRNOG00000006916; Rattus norvegicus.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from direct assay from UniProtKB).
GO:0004047; Molecular function: aminomethyltransferase activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from direct assay from UniProtKB).
GO:0008480; Molecular function: sarcosine dehydrogenase activity (inferred from electronic annotation from EC).
GO:0042426; Biological process: choline catabolic process (non-traceable author statement from UniProtKB).
GO:0006546; Biological process: glycine catabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR006076; FAD-dep_OxRdtase.
IPR013977; GCV_T_C.
IPR006222; GCV_T_N.
Graphical view of domain structure.
Pfam PF01266; DAO; 1.
PF01571; GCV_T; 1.
PF08669; GCV_T_C; 1.
Pfam graphical view of domain structure.
ProtoNet Q64380.
Genome annotation databases
Ensembl ENSRNOG00000006916; Rattus norvegicus. [Contig view]
GeneID 114123; -.
KEGG rno:114123; -.
Phylogenomic databases
HOVERGEN Q64380; -.
Other
NextBio 618353; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   919        Sarcosine dehydrogenase, mitochondrial. PRO_0000010772
MOD_RES   109   109        Tele-8alpha-FAD histidine (By similarity). 
MOD_RES   778   778        Phosphotyrosine (By similarity). 
MOD_RES   885   885        N6-acetyllysine (By similarity). 
CONFLICT   92   102        GGVVLLERERL -> SGVGAAGERAM (in Ref. 1; AAB03674/AAB04667). 
CONFLICT   114   115        LW -> GR (in Ref. 2; AA sequence). 
CONFLICT   154   154        L -> F (in Ref. 1; AAB03674/AAB04667). 
Sequence information
Length: 919 AA [This is the length of the unprocessed precursor] Molecular weight: 101440 Da [This is the MW of the unprocessed precursor] CRC64: E49E54207BD1DBFB [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MASLSRVLRV AATCPRGRSA RNLGLQPLAR EARPTTEKSV PYQCTLKEEA QGASVVPQGP 

        70         80         90        100        110        120 
SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGVVLLERE RLTSGTTWHT AGLLWQLRPS 

       130        140        150        160        170        180 
DVEVELLAHT RQVVSHDLEE ETGLHTGWIQ NGGLFIASNQ QRLDEYKRLM SLGKAYGIES 

       190        200        210        220        230        240 
HVLSPAETKD LYPLMNVDDL YGTLYVPRDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 

       250        260        270        280        290        300 
IRVRTDDFGV RRVTAVETQH GSIQTPCVVN CAGVWASSVG RMAGVKVPLV AMHHAYVVTE 

       310        320        330        340        350        360 
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWD EVSDKFAFGL FDLDWDVFTQ 

       370        380        390        400        410        420 
HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 

       430        440        450        460        470        480 
CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHPRWIRER SHESYAKNYS VVFPHDEPLA 

       490        500        510        520        530        540 
GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG HQAHKDYAYS 

       550        560        570        580        590        600 
RLLGDEYTFD FPPHHCVIQK ECLACRTAAA VFNMSYFGKF YLLGADARKA PDWLFSADVN 

       610        620        630        640        650        660 
RPPGSTVYTC MLNQRGGTES DLTVSCLAPG AQASPLAPAF EGDGYYLAVG GAVAQHNWSH 

       670        680        690        700        710        720 
INTVLQDQEF RCQLMDCSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 

       730        740        750        760        770        780 
HLVRAIRLSF VGELGWELHV PQASCLPVYR AVMAAGAKHG LVNAGYRAID SLSIEKGYRH 

       790        800        810        820        830        840 
WHADLRSDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLVCLT VEEEVPMFGL 

       850        860        870        880        890        900 
EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGDYA LERMGVTYAA 

       910 
QVHLKSPFDP DNKRVKGIY
Q64380 in FASTA format

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