[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). TISSUE=Kidney; The German cDNA consortium; Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.
CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID Q5RF11-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID Q5RF11-2

Features which should be applied to build the isoform sequence: VSP_031988.
SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR857350; CAH89646.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR859187; CAH91375.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR860920; CAH93025.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001127410.1; -.

3D structure databases

ModBase

Q5RF11.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0047988;	Molecular function: hydroxyacid-oxoacid transhydrogenase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0046872;	Molecular function: metal ion binding (inferred from electronic annotation from InterPro).
GO:0015993;	Biological process: molecular hydrogen transport (inferred from sequence or structural similarity from UniProtKB).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PROSITE

PS00913; ADH_IRON_1; FALSE_NEG.
PS00060; ADH_IRON_2; FALSE_NEG.

ProtoNet

Q5RF11.

Genome annotation databases

GeneID

100174480; -.

Phylogenomic databases

HOVERGEN

Q5RF11; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Mitochondrion; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 467`		`Hydroxyacid-oxoacid transhydrogenase, mitochondrial.`	`PRO_0000322998`
`VAR_SEQ`	`1 48`		`Missing (in isoform 2).`	`VSP_031988`
`CONFLICT`	`60 60`		`V -> A (in Ref. 1; CAH91375).`
`CONFLICT`	`147 147`		`M -> K (in Ref. 1; CAH93025/CAH91375).`
`CONFLICT`	`163 163`		`Y -> H (in Ref. 1; CAH93025).`
`CONFLICT`	`254 254`		`L -> P (in Ref. 1; CAH91375).`

Sequence information

Length: 467 AA [This is the length of the unprocessed precursor]

Molecular weight: 50260 Da [This is the MW of the unprocessed precursor]

CRC64: CFC3D34BF6F65B6C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAASRARVA YLLRQLQRAA CQCPTHSHTY SQAPGLSPSG KTTDYAFEMA VSNIRYGAGV 

        70         80         90        100        110        120 
TKEVGMDLQN MGAKNVCLMT DKNLSKLPPV QVAMDSLVKN GIPFTVYDNV RVEPTDASFM 

       130        140        150        160        170        180 
EAIEFAQKGA FDAYVAVGGG STMDTCMAAN LYASSPHSDF LDYVSAPIGK GKPVSVPLKP 

       190        200        210        220        230        240 
LIAVPTTSGT GSETTGVAIF DYEHLKVKIG IASRAIKPTL GLIDPLHTLH MPARVVANSG 

       250        260        270        280        290        300 
FDVLCHALES YTTLPYHLRS PCPSNPITRP AYQGSNPISD IWAIHALRIV AKYLKRAVRN 

       310        320        330        340        350        360 
PDDLEARSHM HLASAFAGIG FGNAGVHLCH GMSYPISGLV KTYKAKDYNV DHPLVPHGLS 

       370        380        390        400        410        420 
VVLTSPAVFT FTAQMFPERH LETAEILGAD TRTARIQDAG LVLADTLRKF LFDLDVDDGL 

       430        440        450        460 
AAVGYSKADI PALVKGTLPQ ERVTKLAPRP QSEEDLAALF EASMKLY

Q5RF11 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools