[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; NIH - Xenopus Gene Collection (XGC) project; Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism (By similarity).
CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD⁺ + H⁺ + 2 ferrocytochrome b5.
COFACTOR: FAD (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC087294; AAH87294.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001088670.1; -.

UniGene

Xl.49849

3D structure databases

ModBase

Q5PQA4.

Organism-specific databases

Xenbase

XB-FEAT-1006040; -.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004128;	Molecular function: cytochrome-b5 reductase activity (inferred from electronic annotation from EC).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0016126;	Biological process: sterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Pfam

PF00970; FAD_binding_6; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00406; CYTB5RDTASE.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q5PQA4.

Genome annotation databases

GeneID

495932; -.

KEGG

xla:495932; -.

Phylogenomic databases

HOVERGEN

Q5PQA4; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Lipid synthesis; Membrane; NAD; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 296`	`296`	`NADH-cytochrome b5 reductase 2.`	`PRO_0000287553`
`TRANSMEM`	`2 24`	`23`	`Potential.`
`DOMAIN`	`35 147`	`113`	`FAD-binding FR-type.`
`NP_BIND`	`127 142`	`16`	`FAD (By similarity).`
`NP_BIND`	`166 201`	`36`	`FAD (By similarity).`

Sequence information

Length: 296 AA [This is the length of the unprocessed precursor]

Molecular weight: 32664 Da [This is the MW of the unprocessed precursor]

CRC64: BC391EEE22573505 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLVALAAIGV TVLLFLIKAL GSGAKKAPVT LLDPNAKYPL PLIEKQEISH DTKKFRFGLP 

        70         80         90        100        110        120 
SAEHVLGLPV GQHIYLSAKV NGSLVVRAYT PVSSDEVKGH VDLVVKVYYK NVNPKFPDGG 

       130        140        150        160        170        180 
KMSQHLDSLK IGETIDFRGP NGLLVYKGKG KFAIRPDKKA EPKIKVAKHV GMLAGGTGIT 

       190        200        210        220        230        240 
PMLQLIRQIT QDPNDNTKCY LIFANQTEDD ILLRYELETV AKSHPEQFKL WYTLDRPPQG 

       250        260        270        280        290 
WKYGSGFVTA DMIKEHLPPP SEDVLVLMCG PPPMIQFACQ DNLTKLGYPE AGRFAY

Q5PQA4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools