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UniProtKB/Swiss-Prot entry Q38830


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IAA12_ARATH
Primary accession number Q38830
Secondary accession number Q8LA42
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 61)
Name and origin of the protein
Protein name Auxin-responsive protein IAA12
Synonyms Indoleacetic acid-induced protein 12
Protein BODENLOS
Gene name
Name: IAA12
Synonyms: BDL
OrderedLocusNames: At1g04550
ORFNames: T1G11.20
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=cv. Columbia;
DOI=10.1006/jmbi.1995.0454; PubMed=7658471 [NCBI, ExPASy, EBI, Israel, Japan]
Abel S., Nguyen M.D., Theologis A.;
"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana.";
J. Mol. Biol. 251:533-549(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
[5]
MUTANT BDL, AND INTERACTION WITH ARF5.
DOI=10.1101/gad.229402; PubMed=12101120 [NCBI, ExPASy, EBI, Israel, Japan]
Hamann T., Benkova E., Baeurle I., Kientz M., Juergens G.;
"The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning.";
Genes Dev. 16:1610-1615(2002).
[6]
GENE FAMILY, NOMENCLATURE, AND FUNCTION.
DOI=10.1023/A:1015255030047; PubMed=12036262 [NCBI, ExPASy, EBI, Israel, Japan]
Liscum E., Reed J.W.;
"Genetics of Aux/IAA and ARF action in plant growth and development.";
Plant Mol. Biol. 49:387-400(2002).
[7]
TRANSCRIPTIONAL REPRESSION DOMAIN.
DOI=10.1105/tpc.017384; PubMed=14742873 [NCBI, ExPASy, EBI, Israel, Japan]
Tiwari S.B., Hagen G., Guilfoyle T.J.;
"Aux/IAA proteins contain a potent transcriptional repression domain.";
Plant Cell 16:533-543(2004).
Comments
  • FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that function as repressor of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.
  • SUBUNIT: Homo and heterodimers. Interacts with the auxin response factor ARF5.
  • INTERACTION:
    Q9LPW7:AFB3; NbExp=1; IntAct=EBI-617608, EBI-617501;
    Q9ZR12:GRH1; NbExp=1; IntAct=EBI-617608, EBI-617479;
    Q570C0:TIR1; NbExp=1; IntAct=EBI-617608, EBI-307183;
  • SUBCELLULAR LOCATION: Nucleus.
  • ALTERNATIVE PRODUCTS: 1 named isoform [FASTA] produced by alternative splicing. A number of isoforms are produced. According to EST sequences.
    Name1
    Isoform IDQ38830-1
    This is the isoform sequence displayed in this entry.
  • TISSUE SPECIFICITY: Preferentially expressed in stems, leaves and flowers.
  • INDUCTION: By auxin.
  • DOMAIN: The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.
  • SIMILARITY: Belongs to the Aux/IAA family.
  • SIMILARITY: Contains 1 Aux/IAA-ARF domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U18414; AAC49053.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC002376; AAB80631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF332398; AAG48762.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY065240; AAL38716.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY096535; AAM20185.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY088042; AAM65588.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S58498; S58498.
RefSeq NP_171949.1; -.
UniGene At.10466
3D structure databases
ModBase Q38830.
Protein-protein interaction databases
IntAct Q38830; -.
Organism-specific databases
GeneFarm 3159; 342.
TAIR At1g04550; -.
Gene expression databases
ArrayExpress Q38830; -.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR003311; AUX_IAA.
IPR011525; AuxIAA_ARF_dimer.
Graphical view of domain structure.
Pfam PF02309; AUX_IAA; 1.
Pfam graphical view of domain structure.
PROSITE PS50962; IAA_ARF; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q38830.
Genome annotation databases
GeneID 839495; -.
GenomeReviews CT485782_GR; AT1G04550.
KEGG ath:AT1G04550; -.
NMPDR fig|3702.1.peg.601; -.
Other
ProtoNet Q38830.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Auxin signaling pathway; Complete proteome; Nucleus; Repressor; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   239  239     Auxin-responsive protein IAA12. PRO_0000112843
DOMAIN   125   218  94     Aux/IAA-ARF. 
MOTIF   20    24  5     EAR-like (transcriptional repression). 
MUTAGEN   74    74        P->S: In bdl; gain of function. Affects auxin-related developmental processes. 
CONFLICT   119   119        P -> A (in Ref. 4; AAM65588). 
Sequence information
Length: 239 AA [This is the length of the unprocessed precursor] Molecular weight: 26286 Da [This is the MW of the unprocessed precursor] CRC64: BBAADA3E98B222CA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRGVSELEVG KSNLPAESEL ELGLGLSLGG GAWKERGRIL TAKDFPSVGS KRSAESSSHQ 

        70         80         90        100        110        120 
GASPPRSSQV VGWPPIGLHR MNSLVNNQAM KAARAEEGDG EKKVVKNDEL KDVSMKVNPK 

       130        140        150        160        170        180 
VQGLGFVKVN MDGVGIGRKV DMRAHSSYEN LAQTLEEMFF GMTGTTCREK VKPLRLLDGS 

       190        200        210        220        230 
SDFVLTYEDK EGDWMLVGDV PWRMFINSVK RLRIMGTSEA SGLAPRRQEQ KDRQRNNPV 

Q38830 in FASTA format

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