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UniProtKB/Swiss-Prot entry Q0J8A4


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name G3PC_ORYSJ
Primary accession number Q0J8A4
Secondary accession numbers Q42977 Q6ZK60
Integrated into Swiss-Prot on July 10, 2007
Sequence was last modified on October 3, 2006 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 21)
Name and origin of the protein
Protein name Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
Synonyms EC 1.2.1.12
PP38
Gene name
Name: GAPC
Synonyms: GPC
OrderedLocusNames: Os08g0126300, LOC_Os08g03290
ORFNames: OJ1163_G08.15, OsJ_024858
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Ilpoombyeo;
TISSUE=Seed;
Yoon U.H., Kim Y.H.;
"Molecular cloning of glyceraldehyde-3-phosphate dehydrogenase genes in rice seeds.";
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1038/nature03895; PubMed=16100779 [NCBI, ExPASy, EBI, Israel, Japan]
International rice genome sequencing project (IRGSP);
"The map-based sequence of the rice genome.";
Nature 436:793-800(2005).
[3]
GENOME REANNOTATION.
STRAIN=cv. Nipponbare;
DOI=10.1101/gr.5509507; PubMed=17210932 [NCBI, ExPASy, EBI, Israel, Japan]
The rice annotation project (RAP);
"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana.";
Genome Res. 17:175-183(2007).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1371/journal.pbio.0030038; PubMed=15685292 [NCBI, ExPASy, EBI, Israel, Japan]
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
"The genomes of Oryza sativa: a history of duplications.";
PLoS Biol. 3:266-281(2005).
[5]
PROTEIN SEQUENCE OF 2-11; 30-39 AND 140-149.
STRAIN=cv. Nipponbare;
TISSUE=Anther, Callus, Embryo, Panicle, and Stem;
DOI=10.1093/nar/gkh020; PubMed=14681440 [NCBI, ExPASy, EBI, Israel, Japan]
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003.";
Nucleic Acids Res. 32:D388-D392(2004).
[6]
IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, AND PHOSPHORYLATION.
STRAIN=cv. Nipponbare;
DOI=10.1007/s11103-005-4013-1; PubMed=16028114 [NCBI, ExPASy, EBI, Israel, Japan]
Khan M.M.K., Jan A., Karibe H., Komatsu S.;
"Identification of phosphoproteins regulated by gibberellin in rice leaf sheath.";
Plant Mol. Biol. 58:27-40(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
EF122472; ABL74559.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP003886; BAD08850.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP008214; BAF22811.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CM000145; EAZ41375.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001060897.1; -.
UniGene Os.12168
3D structure databases
HSSP P56649; 1IHX. [HSSP ENTRY / PDB]
ModBase Q0J8A4.
Organism-specific databases
Gramene Q42977; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.
PANTHER PTHR10836; GAP_DH; 1.
Pfam PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000149; GAP_DH; 1.
PRINTS PR00078; G3PDHDRGNASE.
TIGRFAMs TIGR01534; GAPDH-I; 1.
PROSITE PS00071; GAPDH; 1.
BLOCKS Q0J8A4.
ProtoNet Q0J8A4.
Genome annotation databases
GeneID 4344564; -.
KEGG osa:4344564; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   337  336     Glyceraldehyde-3-phosphate dehydrogenase, cytosolic. PRO_0000145609
NP_BIND   13    14  2     NAD (By similarity). 
REGION   153   155  3     Glyceraldehyde 3-phosphate binding (By similarity). 
REGION   213   214  2     Glyceraldehyde 3-phosphate binding (By similarity). 
ACT_SITE   154   154        Nucleophile (By similarity). 
BINDING   35    35        NAD (By similarity). 
BINDING   82    82        NAD; via carbonyl oxygen (By similarity). 
BINDING   184   184        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   236   236        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   318   318        NAD (By similarity). 
SITE   181   181  1     Activates thiol group during catalysis (By similarity). 
Sequence information
Length: 337 AA [This is the length of the unprocessed precursor] Molecular weight: 36413 Da [This is the MW of the unprocessed precursor] CRC64: 3A8154CF7A06A603 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGQWKHSD 

        70         80         90        100        110        120 
IKIKDSKTLL LGEKPVTVFG IRNPDEIPWA EAGAEYVVES TGVFTDKEKA AAHLKGGAKK 

       130        140        150        160        170        180 
VVISAPSKDA PMFVCGVNED KYTSDIDIVS NASCTTNCLA PLAKVIHDNF GIIEGLMTTV 

       190        200        210        220        230        240 
HAITATQKTV DGPSSKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV 

       250        260        270        280        290        300 
DVSVVDLTVR IEKAASYDAI KSAIKSASEG KLKGIIGYVE EDLVSTDFVG DSRSSIFDAK 

       310        320        330 
AGIALNDNFV KLVAWYDNEW GYSNRVIDLI RHMAKTQ 

Q0J8A4 in FASTA format

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