NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Flower bud;
PubMed=8223617 [NCBI, ExPASy, EBI, Israel, Japan]
Britsch L., Dedio J., Saedler H., Forkmann G.;
"Molecular characterization of flavanone 3 beta-hydroxylases. Consensus sequence, comparison with related enzymes and the role of conserved histidine residues.";
Eur. J. Biochem. 217:745-754(1993).

Comments

FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
COFACTOR: Iron.
COFACTOR: Ascorbate.
PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X72592; CAA51190.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X70378; CAA49839.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S31921; S31921.

3D structure databases

HSSP

Q96323; 1GP6. [HSSP ENTRY / PDB]

ModBase

Q05964.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0031418;	Molecular function: L-ascorbic acid binding (inferred from electronic annotation from UniProtKB-KW).
GO:0045486;	Molecular function: naringenin 3-dioxygenase activity (inferred from electronic annotation from EC).
GO:0016702;	Molecular function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (inferred from electronic annotation from UniProtKB-KW).
GO:0009813;	Biological process: flavonoid biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 365`	`365`	`Naringenin,2-oxoglutarate 3-dioxygenase.`	`PRO_0000067285`
`METAL`	`76 76`		`Iron (Potential).`
`METAL`	`218 218`		`Iron (Potential).`
`METAL`	`220 220`		`Iron (Potential).`
`METAL`	`276 276`		`Iron (Potential).`

Sequence information

Length: 365 AA [This is the length of the unprocessed precursor]

Molecular weight: 40964 Da [This is the MW of the unprocessed precursor]

CRC64: 55F39E674A9293C9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVAEKPKTLT SLEGDDKLNS NFVRDEDERP KVAYNEFSND IPVISLAGID GEKRGEICRK 

        70         80         90        100        110        120 
IVEACEDWGI FQVVDHGVGD DLIADMTRLA REFFALPAEE KLRFDMSGGK KGGFIVSSHL 

       130        140        150        160        170        180 
QGEVVQDWRE IVTYFSYPTN SRDYTRWPDK PEGWIKVTEE YSNKLMTLAC TLLGVLSEAM 

       190        200        210        220        230        240 
GLELEALTKA CVDMDQKIVV NYYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD 

       250        260        270        280        290        300 
GGKTWITVQP VPGAFVVNLG DHGHFLSNGR FKNADHQAVV NSECSRLSIA TFQNPSPDAT 

       310        320        330        340        350        360 
VYPLAIREGE NSIMEEPITF ADLYRRKMAK DLEIARHKRL AKEEMPFKEL DEAKFESKSI 


DQILA

Q05964 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools