NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Horst H39;
PubMed=8979339 [NCBI, ExPASy, EBI, Israel, Japan]
Schaap P.J., Mueller Y., Sonnenberg A.S.M., van Griensven L.J.L.D., Visser J.;
"The Agaricus bisporus pruA gene encodes a cytosolic delta 1-pyrroline-5-carboxylate dehydrogenase which is expressed in fruit bodies but not in gill tissue.";
Appl. Environ. Microbiol. 63:57-62(1997).

Comments

CATALYTIC ACTIVITY: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H.
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2 .
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X95584; CAA64836.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P78568.

Ontologies

GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR005931; d-1-pyrroline-5-COlate_DHase-1.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01236; D1pyr5carbox1; 1.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; NAD; Oxidoreductase; Proline metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 546`	`546`	`Delta-1-pyrroline-5-carboxylate dehydrogenase.`	`PRO_0000056501`
`NP_BIND`	`279 284`	`6`	`NAD (By similarity).`
`ACT_SITE`	`297 297`		`Proton acceptor (By similarity).`
`ACT_SITE`	`331 331`		`Nucleophile (By similarity).`
`SITE`	`195 195`	`1`	`Transition state stabilizer (By similarity).`

Sequence information

Length: 546 AA [This is the length of the unprocessed precursor]

Molecular weight: 59757 Da [This is the MW of the unprocessed precursor]

CRC64: 6B795E09AC242945 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATAQLATFK IPPVSNEPML SYAPGSPERA GLQAALAEMQ SQLPFEVPCI INGQEVRTNN 

        70         80         90        100        110        120 
IQKQPMPHDH ARHLCTFHEG SPELVEKATC GALQAKDGWE TMPWNDRAAI FLKAADLASG 

       130        140        150        160        170        180 
KYRYKLMAAT MLGQGKNTWQ AEIDAAAELA DFFRFGVSYV EELYAQQPPK NAPGCWNRTE 

       190        200        210        220        230        240 
YRPLEGFVLA VSPFNFTAIG GNLPGSPALV GNVVVWKPAP AATYSNYLVF KILSEAGVPP 

       250        260        270        280        290        300 
GVIQFIPGGA EIVQAAIQSP NFRSLHFTGS TNVFKSLWKD ISSNLDKYKV YPRIVGETGG 

       310        320        330        340        350        360 
KNWHVIHKSA EVRNAVLQSV RGAFEYQGQK CSALSRLYVS RSVWENGFKT QYLEEIAKIK 

       370        380        390        400        410        420 
VGPCLDWNNY MGPVIGRRAY DNITGFIKKA KEEGGEVLIG GSGDDSKGFF IQPTVILTKV 

       430        440        450        460        470        480 
PRSTTMVGEI FGPVVTAYVF EDSDYEKTLE LIDTTSIYGL TGAIFASERQ ALLTATNRSR 

       490        500        510        520        530        540 
NAAGNIYYNE KCTGAVVGQQ PFGGARGSGT NDKAGSISIF YRFVSARSIK ENFVGLEDFH 


YPSNLV

P78568 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools