[1]	PROTEIN SEQUENCE. TISSUE=Venom; DOI=10.1074/jbc.270.38.22361; PubMed=7673220 [NCBI, ExPASy, EBI, Israel, Japan] Hopkins C., Grilley M., Miller C., Shon K.-J., Cruz L.J., Gray W.R., Dykert J., Rivier J.E., Yoshikami D., Olivera B.M.; "A new family of Conus peptides targeted to the nicotinic acetylcholine receptor."; J. Biol. Chem. 270:22361-22367(1995).
[2]	SYNTHESIS, AND FUNCTION. DOI=10.1021/bi052016d; PubMed=16430227 [NCBI, ExPASy, EBI, Israel, Japan] Teichert R.W., Lopez-Vera E., Gulyas J., Watkins M., Rivier J., Olivera B.M.; "Definition and characterization of the short alphaA-conotoxins: a single residue determines dissociation kinetics from the fetal muscle nicotinic acetylcholine receptor."; Biochemistry 45:1304-1312(2006).
[3]	STRUCTURE BY NMR, AND DISULFIDE BONDS. DOI=10.1021/bi962301k; PubMed=9048550 [NCBI, ExPASy, EBI, Israel, Japan] Han K.-H., Hwang K.-J., Kim S.-M., Kim S.-K., Gray W.R., Olivera B.M., Rivier J.E., Shon K.-J.; "NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA."; Biochemistry 36:1669-1677(1997).

Comments

FUNCTION: Alpha-A-long conotoxins act on postsynaptic membranes, they bind with high affinity to both fetal (alpha-1/beta-1/epsilon/delta subunits) and adult (alpha-1/beta-1/gamma/delta subunits) mammalian muscle nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin has higher affinity for the adult subtype (IC₅₀=2.3 nM) of the receptor than for the fetal subtype (IC₅₀=22 nM).
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed by the venom duct.
SIMILARITY: Belongs to the conotoxin A superfamily. Alpha-A-type family. Alpha-A-long subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

A58647; A58647.

3D structure databases

PDB

1P1P; NMR; -; A=1-25.

[ExPASy / RCSB / EBI]

PDBsum

1P1P; -.

ModBase

P55963.

Family and domain databases

InterPro

IPR012498; Toxin_14.
Graphical view of domain structure.

Pfam

PF07829; Toxin_14; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Acetylcholine receptor inhibitor; Amidation; Direct protein sequencing; Hydroxylation; Neurotoxin; Postsynaptic neurotoxin; Secreted; Toxin.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`PEPTIDE`	`1 25`	`25`	`Alpha-A conotoxin PIVA.`	`PRO_0000044466`
`MOD_RES`	`7 7`		`4-hydroxyproline; partial.`
`MOD_RES`	`13 13`		`4-hydroxyproline; partial.`
`MOD_RES`	`20 20`		`4-hydroxyproline.`
`MOD_RES`	`25 25`		`Glutamine amide.`
`DISULFID`	`2 16`
`DISULFID`	`3 11`
`DISULFID`	`14 23`
`TURN`	`13 15`	`3`
`HELIX`	`21 23`	`3`

Sequence information

Length: 25 AA [This is the length of the unprocessed precursor]

Molecular weight: 2608 Da [This is the MW of the unprocessed precursor]

CRC64: 9E2147898D697640 [This is a checksum on the sequence]

        10         20 
GCCGSYPNAA CHPCSCKDRP SYCGQ

P55963 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools