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UniProtKB/Swiss-Prot entry P40012


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PPOX_YEAST
Primary accession number P40012
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 64)
Name and origin of the protein
Protein name Protoporphyrinogen oxidase
Synonyms PPO
EC 1.3.3.4
Gene name
Name: HEM14
OrderedLocusNames: YER014W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.271.15.9120; PubMed=8621563 [NCBI, ExPASy, EBI, Israel, Japan]
Camadro J.-M., Labbe P.;
"Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides.";
J. Biol. Chem. 271:9120-9128(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169868 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
Nature 387:78-81(1997).
[3]
SUBCELLULAR LOCATION.
STRAIN=ATCC 25657 / D273-10B;
PubMed=7798202 [NCBI, ExPASy, EBI, Israel, Japan]
Camadro J.-M., Thome F., Brouillet N., Labbe P.;
"Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein.";
J. Biol. Chem. 269:32085-32091(1994).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z71381; CAA95981.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U18778; AAB64547.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S50472; S50472.
RefSeq NP_010930.1; -.
3D structure databases
ModBase P40012.
Organism-specific databases
CYGD YER014w; -.
SGD S000000816; HEM14.
Yeast-GFP YER014W.
Gene expression databases
GermOnline YER014W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0004729; Molecular function: protoporphyrinogen oxidase activity (inferred from mutant phenotype from SGD).
GO:0006783; Biological process: heme biosynthetic process (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR006076; FAD-dep_OxRdtase.
IPR004572; Proto_IX_ox.
Graphical view of domain structure.
Pfam PF01266; DAO; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00562; proto_IX_ox; 1.
BLOCKS P40012.
ProtoNet P40012.
Proteomic databases
PeptideAtlas P40012; -.
Genome annotation databases
Ensembl YER014W; Saccharomyces cerevisiae. [Contig view]
GeneID 856733; -.
GenomeReviews U00092_GR; YER014W.
KEGG sce:YER014W; -.
NMPDR fig|4932.3.peg.1989; -.
Phylogenomic databases
HOGENOM P40012; -.
Other
LinkHub P40012; -.
NextBio 982849; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; FAD; Flavoprotein; Heme biosynthesis; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Porphyrin biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   539  539     Protoporphyrinogen oxidase. PRO_0000135274
NP_BIND   18    23  6     FAD (Potential). 
MUTAGEN   422   422        L->P: In HEM14-1; loss of activity. 
MUTAGEN   424   424        K->E: In HEM14-1; loss of activity. 
Sequence information
Length: 539 AA [This is the length of the unprocessed precursor] Molecular weight: 59703 Da [This is the MW of the unprocessed precursor] CRC64: 5ECCBD1C033BA5B1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG WIYSCNTRDM 

        70         80         90        100        110        120 
SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI DKGCIADKKF LLDPSDKLVQ 

       130        140        150        160        170        180 
VPNSISTTVK FLLNPLGKGL ITGMMGEWFR KKSPHPGQDE SVESICDRRF GNNYISNNMI 

       190        200        210        220        230        240 
SALLRGIYGD DVSLLSAKRT FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG 

       250        260        270        280        290        300 
CLNDYSNAFG KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV 

       310        320        330        340        350        360 
TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS KLLDEIQSNT 

       370        380        390        400        410        420 
IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF DSVIERNFKP LFDKLSTNPN 

       430        440        450        460        470        480 
ALNKYTKVTA MIGGCMLNEH GVPVVPSREV TINAVKDALN NHLGISNKDL EAGQWEFTIA 

       490        500        510        520        530 
DRCLPRFHVG YDAWQERAER KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK 

P40012 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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