[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). DOI=10.1006/jmbi.1996.0334; PubMed=8676383 [NCBI, ExPASy, EBI, Israel, Japan] Xia Z.-X., Dai W.-W., Zhang Y.-F., White S.A., Boyd G.D., Mathews F.S.; "Determination of the gene sequence and the three-dimensional structure at 2.4 angstroms resolution of methanol dehydrogenase from Methylophilus W3A1."; J. Mol. Biol. 259:480-501(1996).
[2]	PROTEIN SEQUENCE OF 23-53. STRAIN=ATCC 53528 / AS1 / DSM 46235 / LMG 6787 / NCIMB 10515; DOI=10.1016/0167-4838(92)90240-E; PubMed=1311606 [NCBI, ExPASy, EBI, Israel, Japan] Cox J.M., Day D.J., Anthony C.; "The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria."; Biochim. Biophys. Acta 1119:97-106(1992).
[3]	X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). DOI=10.1021/bi00211a002; PubMed=8241148 [NCBI, ExPASy, EBI, Israel, Japan] White S.A., Boyd G.D., Mathews F.S., Xia Z.-X., Dai W.-W., Zhang Y.-F., Davidson V.L.; "The active site structure of the calcium-containing quinoprotein methanol dehydrogenase."; Biochemistry 32:12955-12958(1993).
[4]	X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS). PubMed=1331050 [NCBI, ExPASy, EBI, Israel, Japan] Xia Z.-X., Dai W.-W., Xiong J.-P., Hao Z.-P., Davidson V.L., White S.A., Mathews F.S.; "The three-dimensional structures of methanol dehydrogenase from two methylotrophic bacteria at 2.6-A resolution."; J. Biol. Chem. 267:22289-22297(1992).

Comments

CATALYTIC ACTIVITY: A primary alcohol + acceptor = an aldehyde + reduced acceptor.
SUBUNIT: Heterotetramer composed of 2 alpha and 2 beta subunits.
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Periplasmic side. Note=Periplasmic, but associated with inner membrane.
SIMILARITY: Belongs to the methanol dehydrogenase subunit 2 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U41041; AAA83766.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

PDB

1G72; X-ray; 1.90 A; B/D=23-91.	[ExPASy / RCSB / EBI]
2AD6; X-ray; 1.50 A; B/D=23-91.	[ExPASy / RCSB / EBI]
2AD7; X-ray; 1.50 A; B/D=23-91.	[ExPASy / RCSB / EBI]
2AD8; X-ray; 1.60 A; B/D=23-91.	[ExPASy / RCSB / EBI]
4AAH; X-ray; 2.40 A; B/D=23-91.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1G72; -.
2AD6; -.
2AD7; -.
2AD8; -.
4AAH; -.

ModBase

P38540.

Ontologies

GO:0042597;	Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0018468;	Molecular function: alcohol dehydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR003420; Meth_DHase_bsu.
Graphical view of domain structure.

Gene3D

G3DSA:4.10.160.10; Meth_DH_beta; 1.

Pfam

PF02315; MDH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF029163; Meth_DH_beta; 1.

Other

P38540; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Cell inner membrane; Cell membrane; Direct protein sequencing; Membrane; Methanol utilization; Oxidoreductase; Periplasm; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 22`	`22`
`CHAIN`	`23 91`	`69`	`Methanol dehydrogenase subunit 2.`	`PRO_0000025570`
`DISULFID`	`28 34`
`CONFLICT`	`27 27`		`N -> T (in Ref. 2; AA sequence).`
`CONFLICT`	`31 31`		`P -> A (in Ref. 2; AA sequence).`
`CONFLICT`	`37 37`		`N -> A (in Ref. 2; AA sequence).`
`HELIX`	`56 59`	`4`
`HELIX`	`61 78`	`18`
`HELIX`	`80 83`	`4`

Sequence information

Length: 91 AA [This is the length of the unprocessed precursor]

Molecular weight: 10037 Da [This is the MW of the unprocessed precursor]

CRC64: 0123300087E6716A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKHVLTLLAL ASVFAVSNQA LAYDGQNCKE PGNCWENKPG YPEKIAGSKY DPKHDPVELN 

        70         80         90 
KQEESIKAMD ARNAKRIANA KSSGNFVFDV K

P38540 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools