NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(89)90134-7; PubMed=2773323 [NCBI, ExPASy, EBI, Israel, Japan]
Allison R.F., Janda M., Ahlquist P.;
"Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution.";
Virology 172:321-330(1989).

Comments

FUNCTION: RNA-directed RNA polymerase.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M28817; AAA46371.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JU0117; P2WMCC.

RefSeq

NP_613275.1; -.

3D structure databases

ModBase

P20179.

Ontologies

GO:0000166;	Molecular function: nucleotide binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006410;	Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR007610; DUF585.
IPR001788; RNA-dep_RNA_pol_vir-typ.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

Pfam

PF04522; DUF585; 1.
PF00978; RdRP_2; 1.
Pfam graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

962150; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 808`	`808`	`RNA-directed RNA polymerase 2A.`	`PRO_0000083270`
`DOMAIN`	`470 583`	`114`	`RdRp catalytic.`

Sequence information

Length: 808 AA [This is the length of the unprocessed precursor]

Molecular weight: 92937 Da [This is the MW of the unprocessed precursor]

CRC64: D39AFFE56D29B2BE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKFIPEGET YHVPSFQWMF DQTLESDSHH DEAIFVTESI NESGVDTSVE ITADGTLASY 

        70         80         90        100        110        120 
MHAVKPLVED GLLNPPFDQA RWGLCCKNVV DVYDGLLGYR LIPMAEAARM LYLEIDGSFV 

       130        140        150        160        170        180 
DESECDDWRP VDTSDGFTEA MFDVMNEIPG EETKNTCALS LEAESRQAPE TSDMVPSEYT 

       190        200        210        220        230        240 
LADRYVTTRE EFASVDSDYD ISLNLVSPVE FRVGVCEDTY RHSEADDPTM PQYHDRISLK 

       250        260        270        280        290        300 
SLEAAGHHML PTHAYFDDTY YQALEELGDY NVDISKLSVR QSDVDWYRDP EKYYEPELSI 

       310        320        330        340        350        360 
GSFQRRIGTQ KTVLTALKKR NADVPELADS VDIKRVACEV AEKFKRAYLN HSGIGLLGQS 

       370        380        390        400        410        420 
MDVMSRGLEY HKKWKDHKDL TGVTVLSEIN LQRYQHMIKS DIKPVVSDTL HLERAVAATI 

       430        440        450        460        470        480 
TFHGKGVTSC FSPYFTACFE KFSKALKSRF VVPIGKISSL ELKNVPLSNK WFLEADLSKF 

       490        500        510        520        530        540 
DKSQGELHLE FQREILLSLG FPAPLTNWWC DFHRESMLSD PHAGVNMPVS FQRRTGDAFT 

       550        560        570        580        590        600 
YFGNTLVTMA MMAYCCDMNT VDCAIFSGDD SLLICKSKPH LDANVFQSLF NMEIKVMDPS 

       610        620        630        640        650        660 
LPYVCSKFLL ETEMNNLVSV PDPMREIQRL AKRKIIKSPE LLRAHFESFC DRMKFLNKLD 

       670        680        690        700        710        720 
EKMINLLCKF VALKYKKPDV ENDVRVAIAA FGYYSENFLR FCECYATEGV NIYKVKHPIT 

       730        740        750        760        770        780 
QEWFEASRDR DGDWFHDWRN PKFPTALDKV WRFFGKYARD DPMKHIEERD RRHRLNRAMN 

       790        800 
SSLKLAYDRR SLSKDKETVA WVRKTLSK

P20179 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools