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UniProtKB/Swiss-Prot entry P19582


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHOM_BACSU
Primary accession number P19582
Secondary accession numbers O32122 P70991
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on May 30, 2000 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 65)
Name and origin of the protein
Protein name Homoserine dehydrogenase
Synonyms HDH
EC 1.1.1.3
Gene name
Name: hom
Synonyms: tdm
OrderedLocusNames: BSU32260
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=3139660 [NCBI, ExPASy, EBI, Israel, Japan]
Parsot C., Cohen G.N.;
"Cloning and nucleotide sequence of the Bacillus subtilis hom gene coding for homoserine dehydrogenase. Structural and evolutionary relationships with Escherichia coli aspartokinases-homoserine dehydrogenases I and II.";
J. Biol. Chem. 263:14654-14660(1988).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 353-433.
STRAIN=168;
PubMed=3098560 [NCBI, ExPASy, EBI, Israel, Japan]
Parsot C.;
"Evolution of biosynthetic pathways: a common ancestor for threonine synthase, threonine dehydratase and D-serine dehydratase.";
EMBO J. 5:3013-3019(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M23217; AAA50609.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99120; CAB15216.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X04603; CAA28269.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A31973; DEECHS.
RefSeq NP_391106.1; -.
3D structure databases
ModBase P19582.
Enzyme and pathway databases
BioCyc BSUB224308:BSU3223-MON; -.
Organism-specific databases
SubtiList BG10460; hom. [Micado]
Family and domain databases
InterPro IPR002912; ACT_bd.
IPR005106; Asp/hSer_DHase_NAD-bd.
IPR016204; hSer_DHase.
IPR001342; hSer_DHase_cat.
Graphical view of domain structure.
Pfam PF01842; ACT; 1.
PF00742; Homoserine_dh; 1.
PF03447; NAD_binding_3; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000098; Homoser_dehydrog; 1.
PROSITE PS01042; HOMOSER_DHGENASE; 1.
BLOCKS P19582.
Genome annotation databases
GeneID 936654; -.
GenomeReviews AL009126_GR; BSU32260.
KEGG bsu:BSU32260; -.
NMPDR fig|224308.1.peg.3232; -.
Phylogenomic databases
HOGENOM P19582; -.
Genome annotation databases
CMR P19582; BSU32260.
Other
ProtoNet P19582.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Isoleucine biosynthesis; Methionine biosynthesis; NADP; Oxidoreductase; Threonine biosynthesis.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   433  433     Homoserine dehydrogenase. PRO_0000066692
DOMAIN   349   424  76     ACT. 
NP_BIND   9    16  8     NADP (By similarity). 
ACT_SITE   205   205        Proton donor (Potential). 
BINDING   105   105        NADP (By similarity). 
BINDING   190   190        Substrate (By similarity). 
CONFLICT   375   375        S -> T (in Ref. 3; CAA28269). 
CONFLICT   402   402        E -> Q (in Ref. 1; AAA50609). 
Sequence information
Length: 433 AA [This is the length of the unprocessed precursor] Molecular weight: 47494 Da [This is the MW of the unprocessed precursor] CRC64: 03E9DF7727D62696 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKAIRVGLLG LGTVGSGVVK IIQDHQDKLM HQVGCPVTIK KVLVKDLEKK REVDLPKEVL 

        70         80         90        100        110        120 
TTEVYDVIDD PDVDVVIEVI GGVEQTKQYL VDALRSKKHV VTANKDLMAV YGSELLAEAK 

       130        140        150        160        170        180 
ENGCDIYFEA SVAGGIPILR TLEEGLSSDR ITKMMGIVNG TTNFILTKMI KEKSPYEEVL 

       190        200        210        220        230        240 
KEAQDLGFAE ADPTSDVEGL DAARKMAILA RLGFSMNVDL EDVKVKGISQ ITDEDISFSK 

       250        260        270        280        290        300 
RLGYTMKLIG IAQRDGSKIE VSVQPTLLPD HHPLSAVHNE FNAVYVYGEA VGETMFYGPG 

       310        320        330        340        350        360 
AGSMPTATSV VSDLVAVMKN MRLGVTGNSF VGPQYEKNMK SPSDIYAQQF LRIHVKDEVG 

       370        380        390        400        410        420 
SFSKITSVFS ERGVSFEKIL QLPIKGHDEL AEIVIVTHHT SEADFSDILQ NLNDLEVVQE 

       430 
VKSTYRVEGN GWS 

P19582 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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