ID   4CL1_ORYSJ              Reviewed;         564 AA.
AC   P17814; Q0J6Z8; Q6ZKV9;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 2.
DT   04-NOV-2008, entry version 60.
DE   RecName: Full=Probable 4-coumarate--CoA ligase 1;
DE            Short=4CL 1;
DE            Short=Os4CL1;
DE            EC=6.2.1.12;
DE   AltName: Full=4-coumaroyl-CoA synthase 1;
GN   Name=4CL1; Synonyms=4CL;
GN   OrderedLocusNames=Os08g0245200, LOC_Os08g14760;
GN   ORFNames=OJ1033_B09.16;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91045096; PubMed=2235510; DOI=10.1093/nar/18.20.6144;
RA   Zhao Y., Kung S.D., Dube S.K.;
RT   "Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1.";
RL   Nucleic Acids Res. 18:6144-6144(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17210932; DOI=10.1101/gr.5509507;
RG   The rice annotation project (RAP);
RT   "Curated genome annotation of Oryza sativa ssp. japonica and
RT   comparative genome analysis with Arabidopsis thaliana.";
RL   Genome Res. 17:175-183(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22752273; PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18627494; DOI=10.1111/j.1469-8137.2008.02534.x;
RA   de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J.,
RA   Hamberger B., Douglas C.J.;
RT   "Genome-wide analysis of a land plant-specific acyl:coenzymeA
RT   synthetase (ACS) gene family in Arabidopsis, poplar, rice and
RT   Physcomitrella.";
RL   New Phytol. 0:0-0(2008).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-coumarate + CoA = AMP + diphosphate +
CC       4-coumaroyl-CoA.
CC   -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC       biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step
CC       1/2.
CC   -!- INDUCTION: By fungal elicitor and UV irradiation.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are
CC       involved in the substrate recognition, and are sufficient to
CC       confer the substrate specificity.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA36850.1; Type=Erroneous gene model prediction;
CC       Sequence=CAA36850.1; Type=Frameshift; Positions=496;
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DR   EMBL; X52623; CAA36850.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP003859; BAD05189.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP008214; BAF23267.1; -; Genomic_DNA.
DR   EMBL; AK069932; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; JU0311; JU0311.
DR   RefSeq; NP_001061353.1; -.
DR   UniGene; Os.52921; -.
DR   HSSP; P08659; 1LCI.
DR   GeneID; 4345054; -.
DR   KEGG; osa:4345054; -.
DR   Gramene; P17814; -.
DR   GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   Pfam; PF00501; AMP-binding; 1.
DR   PRINTS; PR00154; AMPBINDING.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Ligase; Nucleotide-binding; Phenylpropanoid metabolism.
FT   CHAIN         1    564       Probable 4-coumarate--CoA ligase 1.
FT                                /FTId=PRO_0000193031.
FT   REGION      282    351       SBD1.
FT   REGION      352    419       SBD2.
FT   CONFLICT    405    405       P -> R (in Ref. 1; CAA36850).
FT   CONFLICT    477    477       I -> N (in Ref. 1; CAA36850).
SQ   SEQUENCE   564 AA;  60902 MW;  9725A4B4A72F8C6E CRC64;
     MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV AARPCLIDGA
     TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN SPEFVLSFFA ASRVGAAVTT
     ANPMSTPHEI ESQLAAAGAT VVITESMAAD KLPSHSHGAL TVVLIDERRD GCLHFWDDLM
     SEDEASPLAG DEDDEKVFDP DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN
     IGLHAGDVIL CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL
     VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG QGYGMTEAGP
     VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL GRNLPGEICI RGQQIMKGYL
     NNPEATKNTI DAEGWLHTGD IGYVDDDDEI FIVDRLKEII KYRGFQVAPA ELEALLITHP
     SIADAAVVGK QIEPEIGEIP VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI
     PKAPSGKILR KELRKQLQHL QQEA
//