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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 33-37.
STRAIN=Wistar;
TISSUE=Liver;
DOI=10.1016/0014-5793(90)80172-F; PubMed=2318307 [NCBI, ExPASy, EBI, Israel, Japan]
Usui E.,
Noshiro M.,
Okuda K.;
"Molecular cloning of cDNA for vitamin D3 25-hydroxylase from rat liver mitochondria.";
FEBS Lett. 262:135-138(1990).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=2175615 [NCBI, ExPASy, EBI, Israel, Japan]
Su P.,
Rennert H.,
Shayiq R.M.,
Yamamoto R.,
Zheng Y.-M.,
Addya S.,
Strauss J.F. III,
Avadhani N.G.;
"A cDNA encoding a rat mitochondrial cytochrome P450 catalyzing both the 26-hydroxylation of cholesterol and 25-hydroxylation of vitamin D3: gonadotropic regulation of the cognate mRNA in ovaries.";
DNA Cell Biol. 9:657-665(1990).
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[3]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=1733943 [NCBI, ExPASy, EBI, Israel, Japan]
Shayiq R.M.,
Avadhani N.G.;
"Sequence complementarity between the 5'-terminal regions of mRNAs for rat mitochondrial cytochrome P-450c27/25 and a growth hormone-inducible serine protease inhibitor. A possible gene overlap.";
J. Biol. Chem. 267:2421-2428(1992).
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[4]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1021/bi00042a003; PubMed=7577965 [NCBI, ExPASy, EBI, Israel, Japan]
Mullick J.,
Addya S.,
Sucharov C.,
Avadhani N.G.;
"Localization of a transcription promoter within the second exon of the cytochrome P-450c27/25 gene for the expression of the major species of two-kilobase mRNA.";
Biochemistry 34:13729-13742(1995).
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[5]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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- FUNCTION: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
- CATALYTIC ACTIVITY: 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADPH + O2 = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-alpha,26-tetraol + NADP+ + H2O.
- COFACTOR: Heme group (By similarity).
- PATHWAY: Hormone biosynthesis; cholecalciferol biosynthesis.
- SUBCELLULAR LOCATION: Mitochondrion membrane.
- TISSUE SPECIFICITY: Expressed in liver, hepatoma, kidney, ovary and epithelial cells of blood vessels.
- SIMILARITY: Belongs to the cytochrome P450 family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 533 AA [This is the length of the unprocessed precursor] |
Molecular weight: 60733 Da [This is the MW of the unprocessed precursor] |
CRC64: DC9153BEB6471D63 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MAVLSRMRLR WALLDTRVMG HGLCPQGARA KAAIPAALRD HESTEGPGTG QDRPRLRSLA
70 80 90 100 110 120
ELPGPGTLRF LFQLFLRGYV LHLHELQALN KAKYGPMWTT TFGTRTNVNL ASAPLLEQVM
130 140 150 160 170 180
RQEGKYPIRD SMEQWKEHRD HKGLSYGIFI TQGQQWYHLR HSLNQRMLKP AEAALYTDAL
190 200 210 220 230 240
NEVISDFIAR LDQVRTESAS GDQVPDVAHL LYHLALEAIC YILFEKRVGC LEPSIPEDTA
250 260 270 280 290 300
TFIRSVGLMF KNSVYVTFLP KWSRPLLPFW KRYMNNWDNI FSFGEKMIHQ KVQEIEAQLQ
310 320 330 340 350 360
AAGPDGVQVS GYLHFLLTKE LLSPQETVGT FPELILAGVD TTSNTLTWAL YHLSKNPEIQ
370 380 390 400 410 420
EALHKEVTGV VPFGKVPQNK DFAHMPLLKA VIKETLRLYP VVPTNSRIIT EKETEINGFL
430 440 450 460 470 480
FPKNTQFVLC HYVVSRDPSV FPEPESFQPH RWLRKREDDN SGIQHPFGSV PFGYGVRSCL
490 500 510 520 530
GRRIAELEMQ LLLSRLIQKY EVVLSPGMGE VKSVSRIVLV PSKKVSLRFL QRQ
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P17178 in FASTA format |
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