[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. DOI=10.1021/bi00450a031; PubMed=2514801 [NCBI, ExPASy, EBI, Israel, Japan] Runswick M.J., Gennis R.B., Fearnley I.M., Walker J.E.; "Mitochondrial NADH:ubiquinone reductase: complementary DNA sequence of the import precursor of the bovine 75-kDa subunit."; Biochemistry 28:9452-9459(1989).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Fetal pons; NIH - Mammalian Gene Collection (MGC) project; Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of 45 different subunits.
SUBCELLULAR LOCATION: Mitochondrion inner membrane.
SIMILARITY: Belongs to the complex I 75 kDa subunit family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J02877; AAA30662.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC120069; AAI20070.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A33552; A33552.

NP_777245.1; -.

3D structure databases

ModBase

P15690.

Ontologies

GO:0005758;	Cellular component: mitochondrial intermembrane space (inferred from sequence or structural similarity from UniProtKB).
GO:0005747;	Cellular component: mitochondrial respiratory chain complex I (inferred from sequence or structural similarity from UniProtKB).
GO:0005515;	Molecular function: protein binding (inferred from sequence or structural similarity from UniProtKB).
GO:0006915;	Biological process: apoptosis (inferred from sequence or structural similarity from UniProtKB).
GO:0046034;	Biological process: ATP metabolic process (inferred from sequence or structural similarity from UniProtKB).
GO:0045333;	Biological process: cellular respiration (inferred from sequence or structural similarity from UniProtKB).
GO:0006800;	Biological process: oxygen and reactive oxygen species metabolic process (inferred from sequence or structural similarity from UniProtKB).
GO:0051881;	Biological process: regulation of mitochondrial membrane potential (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001041; Ferredoxin.
IPR006656; Mopterin_OxRdtase.
IPR000283; NADH_DHase_75KDa_su_CS.
IPR010228; NADH_quinone_OxRdtase_G.
IPR015405; NuoG_C.
Graphical view of domain structure.

Pfam

PF09326; DUF1982; 1.
PF00111; Fer2; 1.
PF00384; Molybdopterin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01973; NuoG; 1.

PROSITE

PS00197; 2FE2S_FER_1; FALSE_NEG.
PS51085; 2FE2S_FER_2; 1.
PS00641; COMPLEX1_75K_1; 1.
PS00642; COMPLEX1_75K_2; 1.
PS00643; COMPLEX1_75K_3; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSBTAG00000021976; Bos taurus. [Contig view]

GeneID

288380; -.

KEGG

bta:288380; -.

Phylogenomic databases

HOVERGEN

P15690; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 4Fe-4S; Acetylation; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 23`	`23`	`Mitochondrion.`
`CHAIN`	`24 727`	`704`	`NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial.`	`PRO_0000019967`
`DOMAIN`	`30 108`	`79`	`2Fe-2S ferredoxin-type.`
`METAL`	`64 64`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`75 75`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`78 78`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`92 92`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`124 124`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`128 128`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`131 131`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`137 137`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`176 176`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`179 179`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`182 182`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`226 226`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`MOD_RES`	`84 84`		`N6-acetyllysine (By similarity).`

Sequence information

Length: 727 AA [This is the length of the unprocessed precursor]

Molecular weight: 79442 Da [This is the MW of the unprocessed precursor]

CRC64: BEAD8AB5D76FC22D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLRIPVRKAL VGLSKSSKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI 

        70         80         90        100        110        120 
PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW NILTNSEKTK KAREGVMEFL 

       130        140        150        160        170        180 
LANHPLDCPI CDQGGECDLQ DQSMMFGSDR SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT 

       190        200        210        220        230        240 
RCIRFASEIA GVDDLGTTGR GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA 

       250        260        270        280        290        300 
RPWETRKTES IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ 

       310        320        330        340        350        360 
RLTEPMVRNE KGLLTHTTWE DALSRVAGML QSFQGNDVAA IAGGLVDAEA LIALKDLLNR 

       370        380        390        400        410        420 
VDSDTLCTEE VFPTAGAGTD LRSNYLLNTT IAGVEEADVV LLVGTNPRFE APLFNARIRK 

       430        440        450        460        470        480 
SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK ILQDIASGSH PFSQVLQEAK KPMVILGSSA 

       490        500        510        520        530        540 
LQRNDGAAIL AAVSNIAQKI RTSSGVTGDW KVMNILHRIA SQVAALDLGY KPGVEAIQKN 

       550        560        570        580        590        600 
PPKMLFLLGA DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE 

       610        620        630        640        650        660 
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GMTLPYDTLD QVRNRLEEVS PNLVRYDDVE 

       670        680        690        700        710        720 
GANYFQQASE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI SRASQTMAKC VKAVTEGAHA 


VEEPSIC

P15690 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools