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UniProtKB/Swiss-Prot entry P12943

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PHNS_DESGI
Primary accession number P12943
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1990
Sequence was last modified on May 1, 1991 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 82)
Name and origin of the protein
Protein name Periplasmic [NiFe] hydrogenase small subunit [Precursor]
Synonyms EC 1.12.2.1
NiFe hydrogenlyase small chain
Gene name
Name: hydA
From
Desulfovibrio gigas [TaxID: 879] 
Taxonomy Bacteria; Proteobacteria; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-69.
PubMed=3322743 [NCBI, ExPASy, EBI, Israel, Japan]
Li C., Peck H.D. Jr., le Gall J., Przybyla A.E.;
"Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas.";
DNA 6:539-551(1987).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
PubMed=2651421 [NCBI, ExPASy, EBI, Israel, Japan]
Voordouw G., Menon N.K., le Gall J., Choi E.S., Peck H.D. Jr., Przybyla A.E.;
"Analysis and comparison of nucleotide sequences encoding the genes for [NiFe] and [NiFeSe] hydrogenases from Desulfovibrio gigas and Desulfovibrio baculatus.";
J. Bacteriol. 171:2894-2899(1989).
[3]
 PROTEIN SEQUENCE OF 25-58.
DOI=10.1016/0006-291X(87)90376-7; PubMed=3322275 [NCBI, ExPASy, EBI, Israel, Japan]
Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H.;
"Identification of three classes of hydrogenase in the genus, Desulfovibrio.";
Biochem. Biophys. Res. Commun. 149:369-377(1987).
[4]
 PROTEIN SEQUENCE OF 25-49.
DOI=10.1016/0300-9084(88)90070-3; PubMed=3134950 [NCBI, ExPASy, EBI, Israel, Japan]
Niviere V., Forget N., Bovier-Lapierre G.E., Bonicel J., Hatchikian C.;
"Isolation, amino acid analysis and N-terminal sequence determination of the two subunits of the nickel-containing hydrogenase of Desulfovibrio gigas.";
Biochimie 70:267-272(1988).
[5]
 X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS).
DOI=10.1038/373580a0; PubMed=7854413 [NCBI, ExPASy, EBI, Israel, Japan]
Volbeda A., Charon M.-H., Piras C., Hatchikian E.C., Frey M., Fontecilla-Camps J.-C.;
"Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas.";
Nature 373:580-587(1995).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M18083; AAA23377.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A32315; HQDVSG.
3D structure databases
PDB
1FRV; X-ray; 2.85 A; A/C=25-288.[ExPASy / RCSB / EBI]
1YQ9; X-ray; 2.35 A; A/B=25-288.[ExPASy / RCSB / EBI]
2FRV; X-ray; 2.54 A; A/C/E/G/I/S=25-288.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1FRV; -.
1YQ9; -.
2FRV; -.
ModBase P12943.
Ontologies
GO
GO:0042597; Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0047806; Molecular function: cytochrome-c3 hydrogenase activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.
Pfam PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000310; NiFe_hyd_ssu; 1.
PRINTS PR00614; NIHGNASESMLL.
TIGRFAMs TIGR00391; hydA; 1.
BLOCKS P12943.
ProtoNet P12943.
Other
LinkHub P12943; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; 3Fe-4S; 4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Periplasm; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    23  23      
CHAIN   24   288  265     Periplasmic [NiFe] hydrogenase small subunit. PRO_0000013416
METAL   41    41        Iron-sulfur 1 (4Fe-4S). 
METAL   44    44        Iron-sulfur 1 (4Fe-4S). 
METAL   136   136        Iron-sulfur 1 (4Fe-4S). 
METAL   172   172        Iron-sulfur 1 (4Fe-4S). 
METAL   209   209        Iron-sulfur 2 (4Fe-4S); via pros nitrogen. 
METAL   212   212        Iron-sulfur 2 (4Fe-4S). 
METAL   237   237        Iron-sulfur 2 (4Fe-4S). 
METAL   243   243        Iron-sulfur 2 (4Fe-4S). 
METAL   252   252        Iron-sulfur 3 (3Fe-4S). 
METAL   270   270        Iron-sulfur 3 (3Fe-4S). 
METAL   273   273        Iron-sulfur 3 (3Fe-4S). 
CONFLICT   25    28        LTAK -> SEMQ (in Ref. 3; AA sequence). 
CONFLICT   47    48        SL -> LV (in Ref. 3; AA sequence). 
CONFLICT   48    48        L -> V (in Ref. 4; AAA23377). 
STRAND   32    37  6      
HELIX   43    49  7      
TURN   52    55  4      
HELIX   56    62  7      
STRAND   65    68  4      
TURN   70    72  3      
HELIX   78    87  10      
STRAND   90   101  12      
HELIX   103   105  3      
STRAND   109   111  3      
HELIX   116   123  8      
HELIX   124   126  3      
STRAND   127   133  7      
HELIX   134   139  6      
HELIX   142   144  3      
HELIX   155   159  5      
HELIX   160   162  3      
STRAND   167   169  3      
STRAND   171   173  3      
HELIX   176   188  13      
HELIX   201   204  4      
STRAND   205   207  3      
HELIX   209   211  3      
HELIX   215   220  6      
HELIX   230   233  4      
HELIX   239   241  3      
HELIX   245   247  3      
HELIX   252   255  4      
TURN   258   260  3      
TURN   263   267  5      
HELIX   278   281  4      
Sequence information
Length: 288 AA [This is the length of the unprocessed precursor] Molecular weight: 30816 Da [This is the MW of the unprocessed precursor] CRC64: 4827967DA110AE94 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKFCTAVAVA MGMGPAFAPK VAEALTAKKR PSVVYLHNAE CTGCSESLLR TVDPYVDELI 

        70         80         90        100        110        120 
LDVISMDYHE TLMAGAGHAV EEALHEAIKG DFVCVIEGGI PMGDGGYWGK VGRRNMYDIC 

       130        140        150        160        170        180 
AEVAPKAKAV IAIGTCATYG GVQAAKPNPT GTVGVNEALG KLGVKAINIA GCPPNPMNFV 

       190        200        210        220        230        240 
GTVVHLLTKG MPELDKQGRP VMFFGETVHD NCPRLKHFEA GEFATSFGSP EAKKGYCLYE 

       250        260        270        280 
LGCKGPDTYN NCPKQLFNQV NWPVQAGHPC IACSEPNFWD LYSPFYSA
P12943 in FASTA format

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