[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Sprague-Dawley; DOI=10.1016/S0167-4781(97)00185-1; PubMed=9473656 [NCBI, ExPASy, EBI, Israel, Japan] Konno R.; "Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases."; Biochim. Biophys. Acta 1395:165-170(1998).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
CATALYTIC ACTIVITY: A D-amino acid + H₂O + O₂ = a 2-oxo acid + NH₃ + H₂O₂.
COFACTOR: FAD.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Peroxisome.
SIMILARITY: Belongs to the DAMOX/DASOX family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB003400; BAA22840.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC088395; AAH88395.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_446078.1; -.

UniGene

Rn.81058

3D structure databases

HSSP

P00371; 1AN9. [HSSP ENTRY / PDB]

SMR

O35078; 1-339.

ModBase

O35078.

Organism-specific databases

RGD

621138; Dao1.

Gene expression databases

ArrayExpress

O35078; -.

GermOnline

ENSRNOG00000000694; Rattus norvegicus.

Ontologies

GO:0005777;	Cellular component: peroxisome (inferred from electronic annotation from UniProtKB-KW).
GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0003884;	Molecular function: D-amino-acid oxidase activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006181; D-amino_acid_oxidase_CS.
IPR006076; FAD-dep_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

PROSITE

PS00677; DAO; 1.

ProtoNet

O35078.

Genome annotation databases

Ensembl

ENSRNOG00000000694; Rattus norvegicus. [Contig view]

GeneID

114027; -.

KEGG

rno:114027; -.

Phylogenomic databases

HOVERGEN

O35078; -.

Other

NextBio

618183; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Oxidoreductase; Peroxisome.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 346`	`346`	`D-amino-acid oxidase.`	`PRO_0000162765`
`NP_BIND`	`3 17`	`15`	`FAD (Potential).`
`MOTIF`	`344 346`	`3`	`Microbody targeting signal.`
`ACT_SITE`	`227 227`		`By similarity.`
`ACT_SITE`	`306 306`		`By similarity.`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 38820 Da [This is the MW of the unprocessed precursor]

CRC64: 2C4697960137A4FE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRVAVIGAGV IGLSTALCIH ERYHPAQPLH MKIYADRFTP FTTSDVAAGL WQPYLSDPSN 

        70         80         90        100        110        120 
PQEAEWNQQT FDHLQSCLHS PNAEKMGLAL ISGYNLFRDE VPDPFWKSTV LGFRKLTPSE 

       130        140        150        160        170        180 
LDMFPDYSYG WFNTSLLLEG KSYLSWLTER LTERGVKFIH RKVASFEEVV RGGVDVIINC 

       190        200        210        220        230        240 
TGVWAGALQA DASLQPGRGQ IIQVEAPWIK HFILTHDPSL GIYNSPYIIP GSKTVTLGGV 

       250        260        270        280        290        300 
FQLGNWSELN SVHDHNTIWK SCCQLEPTLK NARIMGELTG FRPVRPQVRL ERERLRFGSS 

       310        320        330        340 
SAEVIHNYGH GGYGLTIHWG CAMEAANLFG KILEEKNLSR MPPSHL

O35078 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools