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UniProtKB/Swiss-Prot entry O30808

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO2_RHIME
Primary accession number O30808
Secondary accession numbers None
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on May 30, 2000 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 62)
Name and origin of the protein
Protein name NADP-dependent malic enzyme
Synonyms NADP-ME
EC 1.1.1.40
Gene name
Name: tme
OrderedLocusNames: R00394
ORFNames: SMc01126
From
Rhizobium meliloti (Sinorhizobium meliloti) [TaxID: 382] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
STRAIN=RCR2011 / SU47;
DOI=10.1074/jbc.273.15.9330; PubMed=9535928 [NCBI, ExPASy, EBI, Israel, Japan]
Mitsch M.J., Voegele R.T., Cowie A., Oesteras M., Finan T.M.;
"Chimeric structure of the NAD(P)+- and NADP+-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti.";
J. Biol. Chem. 273:9330-9336(1998).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=1021;
DOI=10.1073/pnas.161294398; PubMed=11481430 [NCBI, ExPASy, EBI, Israel, Japan]
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021.";
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
[3]
 CHARACTERIZATION.
DOI=10.1016/S0167-4838(99)00112-0; PubMed=10407149 [NCBI, ExPASy, EBI, Israel, Japan]
Voegele R.T., Mitsch M.J., Finan T.M.;
"Characterization of two members of a novel malic enzyme class.";
Biochim. Biophys. Acta 1432:275-285(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF017444; AAB82460.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL591688; CAC41831.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_384500.1; -.
3D structure databases
ModBase O30808.
Enzyme and pathway databases
BioCyc SMEL266834:SMC01126-MON; -.
Ontologies
GO
GO:0008415; Molecular function: acyltransferase activity (inferred from electronic annotation from InterPro).
GO:0004473; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity (inferred from electronic annotation from EC).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from InterPro).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR012188; ME_PTA.
IPR016040; NAD(P)-bd.
IPR002505; PTA_PTB.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
PF01515; PTA_PTB; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF036684; ME_PTA; 1.
PROSITE PS00331; MALIC_ENZYMES; 1.
ProtoNet O30808.
Genome annotation databases
GeneID 1232028; -.
GenomeReviews AL591688_GR; R00394.
KEGG sme:SMc01126; -.
NMPDR fig|266834.1.peg.1688; -.
Phylogenomic databases
HOGENOM O30808; -.
Genome annotation databases
CMR O30808; R00394.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; Multifunctional enzyme; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   761  761     NADP-dependent malic enzyme. PRO_0000160246
REGION   1   437  437     Malic enzyme. 
REGION   438   761  324     Phosphate acetyltransferase. 
ACT_SITE   104   104        Proton donor (By similarity). 
ACT_SITE   172   172        Proton acceptor (By similarity). 
METAL   146   146        Divalent metal cation (By similarity). 
METAL   147   147        Divalent metal cation (By similarity). 
BINDING   172   172        NAD (By similarity). 
BINDING   297   297        NAD (By similarity). 
CONFLICT   228   228        L -> F (in Ref. 1; AAB82460). 
CONFLICT   609   636        DMPTSEELADIAEEAAGLAKRLGYVPRV -> KHADLRGAG (in Ref. 1; AAB82460). 
CONFLICT   761   761        T -> TRRGSAESRKTAGTSSVAASPDG (in Ref. 1; AAB82460). 
Sequence information
Length: 761 AA [This is the length of the unprocessed precursor] Molecular weight: 82209 Da [This is the MW of the unprocessed precursor] CRC64: A342A5F41F39DBC7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MPGIDKTDRA MTSVTAQEAL DFHSQGRPGK LEISPTKPMA TQRDLSLAYS PGVAVPVKAI 

        70         80         90        100        110        120 
ADDPATAYDY TARGNMVAVI SNGTAILGLG NLGALASKPV MEGKAVLFKR FADVDSIDLE 

       130        140        150        160        170        180 
VDTENVDEFV NCVRFLGPSF GGINLEDIKA PDCFIIEQRL REVMDIPVFH DDQHGTAIIA 

       190        200        210        220        230        240 
AAGLVNALTL TGRDFKTAKL VCNGAGAAAI ACIELIKAMG FNPENIILCD TKGVIYKGRT 

       250        260        270        280        290        300 
DGMNQWKSAH AVETDRRTLA EALDGADVFF GLSAKGALSA DMVRSMGARP IIFAMANPDP 

       310        320        330        340        350        360 
EITPEEVALI RDDAIVATGR SDYPNQVNNV LGFPYIFRGA LDVRASTIND AMKIAAAEAL 

       370        380        390        400        410        420 
ANLAKEDVPD DVAAAYQGNR PRFGPQYIIP VPFDPRLISA IPMAVAKAAM ETGVARKPIE 

       430        440        450        460        470        480 
DLKAYGQQLS ARRDPIASTL QRIVERVRRQ PKRIVFAEGE EVQMMRSAIA YANQQLGTAL 

       490        500        510        520        530        540 
LLGREEVMRE TAEREGIDLD RAGIQIVNAR LSKRVGAYTD FLYSRLQRKG YLFRDVQRLI 

       550        560        570        580        590        600 
NTDRNHFAAS MVALGDADGM VTGLTRNYST ALEDVRRCID PKPGHRVIGV SIALCRGRTV 

       610        620        630        640        650        660 
LVADTAVHDM PTSEELADIA EEAAGLAKRL GYVPRVAMLA YSTFGHPSGE RSERVREAVK 

       670        680        690        700        710        720 
ILDRRRVDFE YDGEMAADVA LNARVMEQYP FCRLSGTANV LVMPAFHSAS ISTKMLQELG 

       730        740        750        760 
GSTVIGPLLV GLDKSVQIAS MSAKDSDLVN LAAIAAYNAG T
O30808 in FASTA format

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