FUNCTION: Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol (By similarity).
CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP⁺ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6 .
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
SIMILARITY: Belongs to the ERG4/ERG24 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y14389; CAA74747.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

O13597.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0050613;	Molecular function: delta14-sterol reductase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0016126;	Biological process: sterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001171; ERG4_ERG24.
Graphical view of domain structure.

Pfam

PF01222; ERG4_ERG24; 1.
Pfam graphical view of domain structure.

PROSITE

PS01017; STEROL_REDUCT_1; 1.
PS01018; STEROL_REDUCT_2; 1.

ProtoNet

O13597.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 512`	`512`	`Delta(14)-sterol reductase.`	`PRO_0000207498`
`TRANSMEM`	`27 47`	`21`	`Potential.`
`TRANSMEM`	`100 120`	`21`	`Potential.`
`TRANSMEM`	`140 160`	`21`	`Potential.`
`TRANSMEM`	`172 192`	`21`	`Potential.`
`TRANSMEM`	`242 262`	`21`	`Potential.`
`TRANSMEM`	`278 298`	`21`	`Potential.`
`TRANSMEM`	`324 344`	`21`	`Potential.`
`TRANSMEM`	`353 373`	`21`	`Potential.`
`TRANSMEM`	`418 438`	`21`	`Potential.`
`TRANSMEM`	`458 478`	`21`	`Potential.`

Sequence information

Length: 512 AA [This is the length of the unprocessed precursor]

Molecular weight: 57578 Da [This is the MW of the unprocessed precursor]

CRC64: 169A756E60C0E963 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MARTKATARK TVPAQQEAHG YEFGGPIGAS LISFGLPIAC YAFGFLCNDV SGCPPPSLLS 

        70         80         90        100        110        120 
PSKLFTPPTL SNKVPWQHAL DTLAAEVGWP GWSGLINTEA VLGVFFWYGL SLLLWVLLPA 

       130        140        150        160        170        180 
HEVEGTELRT GGRLKYRFNA CLSAVTIFVA CAAGTIVRGP DFQVWTFINR NYIQLLTVNI 

       190        200        210        220        230        240 
IIAYALAIYV YLKSFEVKAG NTEQRELAAG GHSGHILYDW YMGRELNPRI TIPFIGEVDI 

       250        260        270        280        290        300 
KSFMELRPGM IGWVLLDLAF AAKQYKSYGY ITDSMRKWTP LLLGIHVLTI HPVIVIISQS 

       310        320        330        340        350        360 
VYVFDALYME PAILTTMDLT TDGFGFMLSF GDLVWVPFIY SIQAKYLSVH PVALGPLYVA 

       370        380        390        400        410        420 
LILTIQATGY YIFRATNNDK NIFRTNPNDP KVAHLKYIET GTGSRLLTTG WWGTARHINY 

       430        440        450        460        470        480 
LGDWLMSWSY CLPTLAAGYK LTPTILFENS RLVSTDGMKG AGIPITYFYM LYFAILLIHR 

       490        500        510 
ERRDEAKCRR KYGAHWEKYC QIVRWRILPG VY

O13597 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools