- In the forward direction, the enzyme from Arthrobacter sp. acts also
on secondary amine dicarboxylates such as
N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine.
- Dehydrogenation forms an imine, which dissociates to the amino acid
and pyruvate.
- In the reverse direction, the enzyme acts also on neutral amino acids
as an amino donor.
- They include L-amino acids such as 2-aminopentanoic acid,
2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine,
O-acetylserine, methionine, isoleucine, valine, phenylalanine,
leucine and alanine.
- The amino acceptors include 2-oxoacids such as pyruvate,
oxaloacetate, glyoxylate and 2-oxobutyrate.
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